Anti-inflammatory Effects of Sanhuang-Siwu-Tang in Lipopolysaccharide-Stimulated RAW264.7 Macrophages and BV2 Microglial Cells

Sanhuang-Siwu-Tang (SST), composed of seven medicinal herbs, is a well-known herbal formula used for the treatment gynecologic diseases. To expand clinical use SST, we explored anti-inflammatory or anti-neuroinflammatory effects SST water extract in lipopolysaccharide-stimulated RAW264.7 macrophages and BV2 microglial cells. According to HPLC analysis, main components were from Scutellariae Radix, Coptidis Rhizoma, Paeoniae Radix. significantly inhibited pro-inflammatory mediators including lipopolysaccharide (LPS)-induced production nitric oxide (NO) prostaglandin E2 (PGE2) as well protein expression inducible synthase (iNOS) cyclooxygenase-2 (COX-2), interleukin-6 (IL-6) tumor necrosis factor-α (TNF-α) LPS-stimulated Furthermore, these mediated by mitogen-activated kinase-related proteins (MAPK) nuclear factor κ-light-chain-enhancer activated B cells (NF-κB)-related proteins. Overall, this study demonstrated that potential therapeutic prevention inappropriate inflammation, neuroinflammation, neurodegenerative